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Warning!

Puhti and Mahti are being decommissioned in stages, and their storage areas will become fully unavailable from 15 October 2026. Clean up unnecessary files and move any data you need to keep by 31 August 2026. See the Roihu data migration guide for instructions on transferring your data to Roihu.

Puhti computing services have been decommissioned and no new jobs are accepted or executed on its compute nodes. Puhti login nodes and storage services are planned to remain available until 15 October 2026.

Roary

Roary is a high-speed standalone pan genome pipeline, which takes annotated assemblies in GFF3 format (produced by e.g. Prokka) and calculates the pan genome.

License

Free to use and open source under GNU GPLv3.

Available

  • Puhti: 3.13.0

Usage

On Puhti, Roary should be executed as a batch job. An interactive batch job for running Roary can be started with the command:

sinteractive -i 

To use Roary, load the module using the command:

module load roary

After that, you can launch Roary with the command roary. For example:

roary -f ./demo -e -n -v ./gff/*.gff

More information