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Warning!

Puhti and Mahti will be decommissioned after Roihu becomes available. Users should clean up unnecessary files and move any required data by the end of August 2026. See the Roihu data preparation instructions for details.

Puhti scratch is very full: keep only active data there and move or delete everything else. No new Puhti scratch quota will be granted.

Roary

Roary is a high-speed standalone pan genome pipeline, which takes annotated assemblies in GFF3 format (produced by e.g. Prokka) and calculates the pan genome.

License

Free to use and open source under GNU GPLv3.

Available

  • Puhti: 3.13.0

Usage

On Puhti, Roary should be executed as a batch job. An interactive batch job for running Roary can be started with the command:

sinteractive -i 

To use Roary, load the module using the command:

module load roary

After that, you can launch Roary with the command roary. For example:

roary -f ./demo -e -n -v ./gff/*.gff

More information