Prokka is a software tool to annotate bacterial, archaeal and viral genomes.
Prokka 1.14.6 is Available in Puhti.
In Puhti, Prokka should be executed as a batch job. An interactive batch job for testing Prokka can be started with command:
sinteractive -i -m 8G
Prokka is installed to Puhti as a bioconda environment called
prokka. In addition to Prokka, this environment
contains also Roary pan genome pipeline.
To use it, you should activate Prokka environment with commands:
export PROJAPPL=/projappl/your_project_name module load bioconda source activate prokka
After that you can launch Prokka with command
prokka. By default Prokka tries to use 8 coputing cores, but in
this interactive batch job case, you have just one core available. Because of that you should always define the number
of cores that Prokka will use with option
prokka --cpus 1 contigs.fasta
Larger analysis should be executed as a batch job utilizing several cores. Sample batch job script (batch_job_file.bash) below.
#!/bin/bash -l #SBATCH --job-name=prokka #SBATCH --output=output_%j.txt #SBATCH --error=errors_%j.txt #SBATCH --time=24:00:00 #SBATCH --ntasks=1 #SBATCH --nodes=1 #SBATCH --cpus-per-task=8 #SBATCH --mem=16000 #SBATCH --account=your_project_name # #set up prokka export PROJAPPL=/projappl/your_project_name module load bioconda source activate prokka #Run prokka prokka --cpus $SLURM_CPUS_PER_TASK --outdir results_case1 --prefix mygenome contigs_case1.fa
In the batch job example above one Prokka task (--ntasks 1) is executed. The job reserves 8 core (--cpus-per-task=8 ) with total of 16 GB of memory (--mem=16000). The maximum duration of the job is twelve hours (--time 24:00:00 ). All the cores are assigned from one computing node (--nodes=1 ). In addition to the resource reservations, you have to define the billing project for your batch job. This is done by replacing the your_project_name with the name of your project. (You can use command csc-workspaces to see what projects you have in Puhti).
You can submit the batch job file to the batch job system with command:
See the Puhti user guide for more information about running batch jobs.